Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

推荐稀释比 WB:1/1000-1/10000; IF:1/100-1/500; IP:1/20

Immunofluorescence analysis of Flag Tag (7E1)) in 293 cells transfected with a Flagtag protein using Flag Tag (7E1) antibody(red),and DAPI (blue).

Immunoprecipitation analysis of Flag Tag (7E1) in 1) untransfected 293 cell lysates 2) transfected 293 cell lysates with Flagtag fusion protein 3) IP (transfected 293+ normal mouse IgG+Protein G agarose) 4) IP (transfected 293+anti Flag mA+Protein G agarose).

Western blot analysis of Flag Tag (7E1) in CHO-K1 cells (C) or CHO-K1 cells transfected with different FLAGfusion proteins (A, B), using FlagTag antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.