A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

推荐稀释比 WB:1/3000; IP:1/200

Western blot analysis of Histag in various bacterial lysates, which containing Histagged proteins (MW: 30 kDa, 45 kDa, 72 kDa, 44 kDa and 40 kDa respectively) using antihis antibody.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.