Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

推荐稀释比 WB:1/500-1/1000; IHC:P:1/100-1/200; ICC/IF:1/200-1/500; FC:1/20-1/50; IP:1/20

Western blot analysis of eIF4EBP1 in mouse heart lysates using 4E BP1 antibody.

Western blot analysis of eIF4EBP1 in Jurkat, C6, CHO-K1 lysates using eIF4EBP1 antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using eIF4EBP1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.