Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 150 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

推荐稀释比 WB:1/5000-1/20000;IHC-P:1/500-1/1000;ELISA:1/5000-1/20000

Western blot analysis of HeLa lysates using GAPDH antibody as the primary antibody, decreasing concentrations of Goat anti-rabbit IgG (H&L) (HRP conjugate) secondary antibodies were used.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using NAT10 antibody. Secondary antibody Goat anti-rabbit IgG (H&L)（HRP conjugate）High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.