Swiss-Prot Acc.C8CHS1.Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization.

推荐稀释比 WB:1/1000-1/10000;IP:1/20

Western blot analysis of EGFP/EYFP in CHO-K1 transfected by different EGFPfusion proteins(MW:A. 30kDa, B. 38kDa, C. 50kDa) or nontransfected (D) lysates using EGFP/EYFP antibody.

Immunoprecipitation analysis of GFP/eGFP Tag in CHO-K1 lysates transfected with EGFPCyclin D2 fragment fusion protein. IP: EGFP/EYFP antibody; WB: Cyclin D2 antibody(200500).

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.