The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008].

推荐稀释比 IHC:1/50-1/100

Immunohistochemistry analysis of paraffin-embedded Human colon tissue within the normal limits using GPA33 antibbody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded Human colon Adenocarcinoma using GPA33 antibbody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.