K10 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200

Western

Western blot analysis of Cytokeratin 10 in A431 lysates using Cytokeratin 10 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human bladder using Cytokeratin 10 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Cytokeratin 10 in HACAT using Cytokeratin 10 antibody.

-15到-25度