Topoisomerase I Rabbit mAb
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38554ES50
50 μL
现货
¥ 1385.00
38554ES60
100 μL
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¥ 2385.00
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Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.
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推荐稀释比 WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
应用案例
Western
Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody.
Immunocytochemistry
Immunocytochemistry analysis of Topoisomerase I(green) in Hela using Topoisomerase I antibody,and DAPI(blue)
Immunohistochemistry
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Topoisomerase I antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
存储条件
-15到-25度
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