Act as a convergence point for multiple signals that control autophagy, and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking. AMPK, activated during low nutrient conditions, directly phosphorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777. Conversely, mTOR, which is a regulator of cell growth and an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK.

Western

Western blot analysis of ULK1 in HEK293 , PC-12 lysates using ULK1 antibody.

Western

Western blot analysis of ULK1 in (1) HEK293 lysates; (2) PC-12 lysates using ULK1 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human heart using ULK1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of ULK1 in 293 using ULK1 antibody.