These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

Western

Western blot analysis of Chk2 in HeLa(WT) and CHEK2-Knockout hela(KO) using Chk2 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CHEK2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of CHEK2 in HT-29 using CHEK2 antibody.

Flow

Flow Cytometry analysis of Jurkat stained with CHEK2 antibody (Red).Blue line histogram represents nonspecific negative control.