Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.

Western

Western blot analysis of Phospho-JAK2 (Tyr1007/1008) in MEF+Pervanadate，C6+Pervanadate， lysates using Phospho-JAK2 (Tyr1007/1008) antibody.

Western

Western blot analysis of JAK2 Phosphorylation in Jurkat lysates treated with Pervanadate using Phospho-JAK2 (Tyr1007/1008) antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using Phospho-JAK2 (Y1007 + Y1008) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Phospho-JAK2 (Tyr1007/1008) in Jurkat treated with Pervanadate using Phospho-JAK2 (Y1007 + Y0018) antibody.