5-Fluorouracil (5-FU)，又称为5-Fluoracil，是一种DNA/RNA合成抑制剂，通过不可逆地抑制胸苷酸合成酶（TS）而干扰核苷酸合成。5-Fluorouracil处理细胞后，引起细胞聚集在S期，并诱导p53依赖的细胞凋亡。在动物肿瘤模型中，5-Fluorouracil与胸苷（thymidine）联合作用时，5-Fluorouracil抗肿瘤作用增强。

5-Fluorouracil已经被用于治疗多种癌症，如结肠癌、乳腺癌和头颈癌等。

参考文献

[1] Van Laar JA, et al. Tumor size and origin determine the antitumor activity of cisplatin or 5-fluorouracil and its modulation by leucovorin in murine colon carcinomas. Cancer Chemother Pharmacol, 39(1-2): 79-89 (1996).

[2] Shi X, et al. Acquired resistance of pancreatic cancer cells towards 5-Fluorouracil and gemcitabine is associated with altered expression of apoptosis-regulating genes. Oncology, 62(4): 354-362 (2002).

[3] Longley DB, et al. 5-fluorouracil: mechanisms of action and clinical strategies. Nat Rev Cancer. 3(5): 330-338 (2003).

[4] Noordhuis P, et al. 5-Fluorouracil incorporation into RNA and DNA in relation to thymidylate synthase inhibition of human colorectal cancers. Ann Oncol 15 (7): 1025-1032 (2004).

[5] Focaccetti C, et al. Effects of 5-Fluorouracil on Morphology, Cell Cycle, Proliferation, Apoptosis, Autophagy and ROS Production in Endothelial Cells and Cardiomyocytes. PLoS ONE 10(2): e0115686 (2015).

冰袋运输。粉末直接保存于-20ºC，有效期2年。溶于DMSO。建议分装后-20ºC避光保存，避免反复冻存，至少可存放6个月。